Spectra viewer

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The SpectraViewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent labelled probes. Revvity Sites Globally Select your location. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, start by selecting the instrument from the Select Machine drop-down menu. The light source and filters for the chosen instrument will be displayed automatically. Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually. Light sources are input in nm format and filters as - Next, select the fluorophores of interest from the Add Fluorophore drop-down menu.

Spectra viewer

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time. Pre-loaded light sources and laser sets for microscopy and flow cytometry applications. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument. Generates a final report listing selected products, all the spectral profiles of fluorophores of interest, together with lasers and filters.

You may remove excitation or emission spectra and filter ranges from the display while keeping them selected by unchecking the spectra viewer boxes.

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Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In. This can be useful for rotating through several overlapping spectra x. Add a spectrum to begin.

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Click here to see all available distributors. For technical assistance on using this web application, please contact websupport aatbio. Note: If a filter is added to the graph, a new column will appear in the information table at the bottom of the page, labeled "Spillover" with the filter shown in parentheses. Under Light Source select the light source line which you want to use to normalize the spectra. To use the viewer for absorbance dyes colorimetric format labels , click the left drop-down menu under "Current mode" and select "Absorbance". Then enter in the bandwidth number into the new input box and click "OK". Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument. Use Print to generate a printed version of your selections and the corresponding spectra. Adding Excitation Sources [ Show ] To add one or more excitation sources, click "Excitation Source" in the "Add" submenu on the left part of the screen. Download and share fluorochrome spectrum alongside laser and filter configurations Generate, export, and share the final report.

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View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Note: if an excitation source is added to the graph, the rightmost column in the information table at the bottom of the page, labeled "Peak Intensity" with the excitation source title in parentheses will show the percentage of the maximum possible intensity for the emission curve of each compound currently on the graph. Use the right-hand scroll bar to view the lower section of the tables if necessary. Navigation Menu. Name Required. Under Light Source select the light source line which you want to use to normalize the spectra. Generates a final report listing selected products, all the spectral profiles of fluorophores of interest, together with lasers and filters. Add a spectrum to begin. If the "Custom" row is selected, a popup box will appear and prompt the user to enter the center wavelength. To exit out of the menu, click the small "x" on the upper right corner of the gray bar at the top of the menu.