N benthamiana

Federal government websites often end in. The site is secure. Nicotiana benthamiana is widely used as a model plant for dicotyledonous angiosperms.

Data are scarce about the influence of basic cultural conditions on growth patterns and overall performance of plants used as heterologous production hosts for protein pharmaceuticals. Higher plants are complex organisms with young, mature, and senescing organs that show distinct metabolic backgrounds and differ in their ability to sustain foreign protein expression and accumulation. Here, we used the transient protein expression host Nicotiana benthamiana as a model to map the accumulation profile of influenza virus hemagglutinin H1, a clinically promising vaccine antigen, at the whole plant scale. Greenhouse-grown plants submitted to different light regimes, submitted to apical bud pruning, or treated with the axillary growth-promoting cytokinin 6-benzylaminopurine were vacuum-infiltrated with agrobacteria harboring a DNA sequence for H1 and allowed to express the viral antigen for 7 days in growth chamber under similar environmental conditions. Our data highlight the importance of young leaves on H1 yield per plant, unlike older leaves which account for a significant part of the plant biomass but contribute little to total antigen titer. These findings underline the relevance of both considering main stem leaves and axillary stem leaves while modeling heterologous protein production in N. They also demonstrate the potential of exogenously applied growth-promoting hormones to modulate host plant architecture for improvement of protein yields.

N benthamiana

Nicotiana benthamiana , colloquially known as benth or benthi , is a species of Nicotiana indigenous to Australia. It is a close relative of tobacco. A synonym for this species is Nicotiana suaveolens var. This was transferred to Nicotiana benthamiana by Karel Domin in Bibliotheca Botanica , honoring the original author in the specific epithet. The plant was used by people of Australia as a stimulant, containing nicotine and other alkaloids , before the introduction of commercial tobacco N. Indigenous names for it include tjuntiwari and muntju. The herbaceous plant is found amongst rocks on hills and cliffs throughout the northern regions of Australia. Variable in height and habit, the species may be erect and up to 1. The flowers are white. For example, the leaves are rather frail and can be injured in experiments to study ethylene synthesis. Ethylene is a plant hormone which is secreted, among other situations, after injuries. Using gas chromatography , the quantity of ethylene emitted can be measured. In , a genetically engineered N. The Quebec City -based biotechnology company, Medicago Inc.

Visualization tools for genomic conservation. Ethylene is a plant hormone which is secreted, among n benthamiana situations, after injuries.

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. An Author Correction to this article was published on 05 January To further improve its usefulness and versatility, we have produced high-quality chromosome-level genome assemblies, coupled with transcriptome, epigenome, microRNA and transposable element datasets, for the ubiquitously used LAB strain and a related wild accession, QLD. In addition, single nucleotide polymorphism maps have been produced for a further two laboratory strains and four wild accessions.

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Nicotiana benthamiana is a workhorse for fundamental research and biotechnology. We assembled and annotated the allotetraploid chromosomal-level genomes and epigenomes of the laboratory strain and a wild strain of Nicotiana benthamiana , creating a reference genome for the genus Nicotiana.

N benthamiana

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

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In almost all of these respects they performed similarly. Methods , 9— Ronquist, F. These plants may partially conserve similar cell wall modifications. Quantitative pathogenicity and host adaptation in a fungal plant pathogen revealed by whole-genome sequencing Article Open access 02 March Gene editing by S. To improve this effort, de novo whole-genome assembly was performed in N. Edgar, S. Siminszky, B. Juicebox provides a visualization system for Hi-C contact maps with unlimited zoom. Wild tobacco genomes reveal the evolution of nicotine biosynthesis. Methods , 1—7. Skip to main content Thank you for visiting nature. Nicotiana benthamiana is one of the most widely used experimental models in plant biology, especially the interaction between different living organisms Bombarely et al. To simplify the gene annotation, only one isoform containing the longest CDS was retained where there appeared to be overlapping genes.

Nicotiana benthamiana is widely used as a model plant for dicotyledonous angiosperms.

Durand, N. As a library, NLM provides access to scientific literature. Dunn, N. Hydroponic treatment of Nicotiana benthamiana with Kifunensine modifies the N -glycans of recombinant glycoprotein antigens to predominantly Man9 high-mannose type upon transient overexpression. The rise and rise of Nicotiana benthamiana : a plant for all reasons. The plants were left to grow in greenhouse for 3 weeks under either light regime before use for leaf agroinfiltration. Accepted : 11 July An Author Correction to this article was published on 05 January The 21 longest scaffolds were regarded as pseudomolecules, which reflected the chromosomes of N. Issue Date : September The genome analysis of a section Noctiflorae , another progenitor of N. Dudchenko, O. Higher plants are complex organisms with young, mature, and senescing organs that show distinct metabolic backgrounds and differ in their ability to sustain foreign protein expression and accumulation. Table 2. Antibodies against two active histone marks, anti-histone-H3-tri-methyl-K4 Abcam, catalogue no.