Cellosaurus

We have developed an online database describing the known cell lines from Coleoptera, cellosaurus, Diptera, Hemiptera, Hymenoptera, and Lepidoptera that were developed from agricultural pests. Cell line information has cellosaurus primarily obtained from previous compilations of insect cell lines. Here, we report on a new cellosaurus of insect cell lines, which covers cell lines from 86 species, cellosaurus. We provide the designation, tissue and species of origin, cell line developer, unique characteristics, its cellosaurus in various applications, publications, and patents, and, when known, insect virus susceptibility.

Cellosaurus is an online knowledge base on cell lines , which attempts to document all cell lines used in biomedical research. Its scope includes immortalised cell lines , naturally immortal cell lines example: embryonic stem cells and finite life cell lines when those are distributed and used widely. The Cellosaurus provides a wealth of manually curated information; for each cell line it lists a recommended name, synonyms and the species of origin. Other types of information include standardised disease terminology for cancer or genetic disorder cell lines , the transformant used to immortalise a cell line, transfected or knocked-out genes, microsatellite instability , doubling time , gender and age of donor patient or animal , important sequence variations, web links, publication references and cross-references to close to different databases, ontologies, cell collections and other relevant resources. Since many cell lines used in research have been misidentified or contaminated , the Cellosaurus keeps track of problematic cell lines, including all those listed in the International Cell Line Authentication Committee ICLAC [8] tables.

Cellosaurus

Despite an increased awareness of the problematic of cell line cross-contamination and misidentification, it remains nowadays a major source of erroneous experimental results in biomedical research. To prevent it, researchers are expected to frequently test the authenticity of the cell lines they are working on. STR profiling was selected as the international reference method to perform cell line authentication. While the experimental protocols and manipulations for generating a STR profile are well described, the available tools and workflows to analyze such data are lacking. The Cellosaurus knowledge resource aimed to improve the situation by compiling all the publicly available STR profiles from the literature and other databases. As a result, it grew to become the largest database in terms of human STR profiles, with 6, distinct cell lines having an associated STR profile release July 31, It aims to help researchers in the process of cell line authentication by providing numerous functionalities. Keywords: STR profiling; authentication; cell culture; cell lines; contamination; misidentification. Abstract Despite an increased awareness of the problematic of cell line cross-contamination and misidentification, it remains nowadays a major source of erroneous experimental results in biomedical research. Substances Biomarkers.

The Cellosaurus knowledge resource cellosaurus to improve the situation by compiling all the publicly available STR profiles from the literature and other databases, cellosaurus.

This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share useful new resources with the scientific community — particularly ones that help address ongoing problems, like cells lines. A study in Science found that approximately one-third of cell lines used in scientific research are misidentified! Rigor and reproducibility of scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus , a growing cell line information database describing nearly , cell lines. Cellosaurus seeks to identify and describe all cell lines used in research, using four methods to search out cell lines:.

Federal government websites often end in. The site is secure. The Cellosaurus is a knowledge resource on cell lines. It aims to describe all cell lines used in biomedical research. Its scope encompasses both vertebrates and invertebrates. For each cell line, it provides a wealth of information, cross-references, and literature citations. Cell lines are ubiquitous tools for experimental biomedical research, both in academic and industrial settings. The mouse L cells were the first immortalized cell line to be established in , 1 but for almost everyone, the dawn of the era of cell lines is associated with the establishment in of the HeLa cell line from the cervical tumor of Henrietta Lacks by George Otto Gey.

Cellosaurus

Cellosaurus is an online knowledge base on cell lines , which attempts to document all cell lines used in biomedical research. Its scope includes immortalised cell lines , naturally immortal cell lines example: embryonic stem cells and finite life cell lines when those are distributed and used widely. The Cellosaurus provides a wealth of manually curated information; for each cell line it lists a recommended name, synonyms and the species of origin. Other types of information include standardised disease terminology for cancer or genetic disorder cell lines , the transformant used to immortalise a cell line, transfected or knocked-out genes, microsatellite instability , doubling time , gender and age of donor patient or animal , important sequence variations, web links, publication references and cross-references to close to different databases, ontologies, cell collections and other relevant resources. Since many cell lines used in research have been misidentified or contaminated , the Cellosaurus keeps track of problematic cell lines, including all those listed in the International Cell Line Authentication Committee ICLAC [8] tables. For human as well as some dog cell lines, it provides short tandem repeat STR profile information. Since July , cell lines in the Cellosaurus are represented as items in Wikidata. The Cellosaurus encyclopedia is widely recognized as an authoritative source for cell line information, providing unique identifiers [10] [11] and as source of curated information. Contents move to sidebar hide.

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Importantly, Cellosaurus also identifies problematic cell lines: ones either suspected to be contaminate or easily contaminated, as well as commonly misidentified lines. Here at Addgene, we like to help share useful new resources with the scientific community — particularly ones that help address ongoing problems, like cells lines. Retrieved Spoiler alert: HeLa is the most common contaminator line. Article Talk. Since July , cell lines in the Cellosaurus are represented as items in Wikidata. The Cellosaurus knowledge resource aimed to improve the situation by compiling all the publicly available STR profiles from the literature and other databases. Animal NA. Abstract Despite an increased awareness of the problematic of cell line cross-contamination and misidentification, it remains nowadays a major source of erroneous experimental results in biomedical research. Cellosaurus seeks to identify and describe all cell lines used in research, using four methods to search out cell lines:.

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If the information seems incomplete or unclear, Bairoch follow up via email to the authors of the paper in which the cell line was described. Rigor and reproducibility of scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus , a growing cell line information database describing nearly , cell lines. Branches of biology. Contributing to Cellosaurus Generated a new cell? The Cellosaurus provides a wealth of manually curated information; for each cell line it lists a recommended name, synonyms and the species of origin. A major contributor to this article appears to have a close connection with its subject. PMID July Learn how and when to remove this template message. STR profiling was selected as the international reference method to perform cell line authentication. Real time : Checking